review of methods to collect settled dust and isolate culturable microorganisms pdf

Review Of Methods To Collect Settled Dust And Isolate Culturable Microorganisms Pdf

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People spend most of their time inside buildings and the indoor microbiome is a major part of our everyday environment. It is still not well understood how environmental conditions affect indoor microbial communities. Existing studies have mostly focussed on the local e.

Genetic analysis of indoor air has uncovered a rich microbial presence, but rarely have both the bacterial and fungal components been examined in the same samples. Here we present a study that examined the bacterial component of passively settled microbes from both indoor and outdoor air over a discrete time period and for which the fungal component has already been reported. We amplified the bacterial 16S rRNA gene in these samples and analyzed them with high-throughput sequencing. Like fungal OTU-richness, bacterial OTU-richness was higher outdoors then indoors and was invariant across different indoor room types. While fungal composition was structured largely by season and residential unit, bacterial composition varied by residential unit and room type.

House dust microbiome and human health risks

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Premina Sivagnanasundaram, R. Amarasekara, R. Madegedara, Anuradha Ekanayake, D. Nosocomial infections, in lay term known as hospital acquired infections, are caused mainly by airborne pathogens found in healthcare facilities and their surroundings. The aim of this study was to quantify and identify bacteria and fungi in a hospital, which is an understudied area of air quality in Sri Lanka. Air samples were collected in agar medium and petri plates containing sterile filter papers.

Sources of airborne microorganisms in the built environment

It is therefore necessary to monitor and control indoor airborne microbes for occupational safety and public health. Most studies concerning airborne microorganisms have focused on fungi, with scant data available concerning bacteria. The present review considers papers published from to approximately and factors affecting properties of indoor airborne bacteria communities and concentration with respect to temporal perspective and to multiscale interaction viewpoint. From a temporal perspective, bacterial concentrations in built environments change depending on numbers of human occupancy, while properties of bacterial communities tend to remain stable. Similarly, the bacteria found in social and community spaces such as offices, classrooms and hospitals are mainly associated with human occupancy. Other major sources of indoor airborne bacteria are i outdoor environments, and ii the building materials themselves.

Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. Culture-independent DNA sequencing of fungal internal transcribed spacer 2 ITS2 region was compared to a culture-dependent morphological identification technique to characterize house dust-borne fungal communities.

Objectives: This study investigated the levels and components of floor-settled dust in two elementary schools located at different sites one near the Shihwa industrial complex and the other in a rural area in order to evaluate the amounts of trace metal elements As, Cd, Co, Cr, Cu, Ni, Pb and Zn and microorganisms. Methods: Over twenty settled-dust samples were collected from the two elementary schools. Trace metal elements were extracted from the dust using hydrochloric acid and nitric acid, and the amounts were measured by ICP-OES. Microbiological analysis was performed by bacterial culturing using R2A medium and denaturing gradient gel electrophoresis DGGE. ScienceON Chatbot. Abstract Objectives: This study investigated the levels and components of floor-settled dust in two elementary schools located at different sites one near the Shihwa industrial complex and the other in a rural area in order to evaluate the amounts of trace metal elements As, Cd, Co, Cr, Cu, Ni, Pb and Zn and microorganisms. Respiratory health effects of indoor air pollution.


Fungi and bacteria are among the many components in dust that have been studied. Isolation by culture is an established method that is used.


Aseptic Laboratory Techniques: Plating Methods

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People spend a lot of time indoors and the indoor microbiome is a major part of the environment that we are exposed to. However, awareness of the exposure to the indoor microbiome and its health effects remains poor. Outdoor environment soil and air , indoor sources ventilation, dampness and building materials , human occupants, and pets compose the indoor microbial community.

Metrics details. Each day people are exposed to millions of bioaerosols, including whole microorganisms, which can have both beneficial and detrimental effects. The next chapter in understanding the airborne microbiome of the built environment is characterizing the various sources of airborne microorganisms and the relative contribution of each. We have identified the following eight major categories of sources of airborne bacteria, viruses, and fungi in the built environment: humans; pets; plants; plumbing systems; heating, ventilation, and air-conditioning systems; mold; dust resuspension; and the outdoor environment. Certain species are associated with certain sources, but the full potential of source characterization and source apportionment has not yet been realized.

Fungi in the Built Environment

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Exposures to bioaerosols in the occupational environment are associated with a wide range of health effects with major public health impact, including infectious diseases, acute toxic effects, allergies and cancer. Respiratory symptoms and lung function impairment are the most widely studied and probably among the most important bioaerosol-associated health effects. In addition to these adverse health effects some protective effects of microbial exposure on atopy and atopic conditions has also been suggested. New industrial activities have emerged in recent years in which exposures to bioaerosols can be abundant, e. Dose—response relationships have not been established for most biological agents and knowledge about threshold values is sparse.

Each day people are exposed to millions of bioaerosols, including whole microorganisms, which can have both beneficial and detrimental effects. The next chapter in understanding the airborne microbiome of the built environment is characterizing the various sources of airborne microorganisms and the relative contribution of each. We have identified the following eight major categories of sources of airborne bacteria, viruses, and fungi in the built environment: humans; pets; plants; plumbing systems; heating, ventilation, and air-conditioning systems; mold; dust resuspension; and the outdoor environment. Certain species are associated with certain sources, but the full potential of source characterization and source apportionment has not yet been realized. Ideally, future studies will quantify detailed emission rates of microorganisms from each source and will identify the relative contribution of each source to the indoor air microbiome.

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Transmission of Airborne Bacteria across Built Environments and Its Measurement Standards: A Review

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